Combined Treatment of Metformin and Resveratrol Promotes Myogenesis Through Increased Irisin Release in C2C12 Cells

초록

PurposeThis study aimed to investigate the additive effects of a combination of metformin and resveratrol on irisin expression in C2C12 cells.MethodsThe study involved treating C2C12 cells with metformin and resveratrol, either alone or in combination, and analyzing their effects on myogenesis and irisin release. The activation of signaling pathways, including AMPK/SIRT1/PGC1 alpha, as well as the relative mRNA and protein expression levels of MyoD, myogenin, and Myh were also assessed.ResultsCombination treatment with metformin and resveratrol significantly increased MyoD, myogenin, Myh, and FNDC5 expression compared with the group treated with metformin alone. The increase in irisin production was associated with phosphorylation of AMPK and upregulation of PGC-1 alpha and SIRT1, indicating activation of the AMPK/SIRT1/PGC-1 alpha pathway. The mRNA and protein expression levels of MyoD, myogenin, and Myh were also significantly higher in the combination treatment group compared to the metformin alone group.ConclusionThe combination of metformin and resveratrol effectively increased irisin release through the AMPK/Sirt1/PGC-1 alpha pathway, suggesting that this combination treatment could enhance myogenesis.

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