Deactivation of Colletotrichum acutatum and Colletotrichum gloeosporioides by novel cylindrical dielectric barrier discharge plasma: Maintaining biochemical composition in strawberry by in-vitro and in-vivo

초록

Strawberry fruits are slightly tender after harvesting, but they can normally fungal spoilage. The key strawberry fruits/plant fungus are Colletotrichum acutatum and Colletotrichum gloeosporioides. There are many alternatives to conservative fungicides that can have a lower impact on humans and the environment, while maintaining the biochemical properties of strawberry fruits/plants. In this study, a multi-cylindrical dielectric barrier discharge plasma (MC-DBDP) system was employed to generate NOx and O3 gas, and plasma-activated water (PAW) by employing 100 % and 26.5 % duty cycle respectively. Study results showed that plasma generated reactive oxygen and nitrogen species (RONS) inactivate the C. acutatum at 2.87 and 3.57 respectively in 10 min, while C. gloeosporiodes was found at ND. Two-way generated NOx was used to inactivate the fungus, PAW-NOx concentration was 250 mu M and PAW-O3 was 30 mu M. C. acutatum decreased by 2.27 and 1.23 in PAW-NOx and PAWO3, respectively, and C. gloeosporioides decreased by 1.23 and 1.42 in PAW-NOx and PAW-O3, respectively. Inactivation of the fungus was achieved by spraying and irrigating strawberry fruits and plants with PAW. PAWNOx not only controls the fungus but also improves biochemical composition of the other treatments (PAW-O3) and control groups. At the same time, the necrotic effect induced by PAW-O3 irrigation is greater when compared to control and PAW-NOx.

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