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Real-time fluorescence assay of DNA polymerase using a graphene oxide platform
산화 그래핀 플랫폼을 이용한 DNA 중합효소의 실시간 형광에세이
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0초록
Using the different adsorption properties of ssDNA and dsDNA to GO, this study used a real time and efficient fluorescence assay to detect the enzymatic activity of the Klenow fragment with the adsorbed DNA to GO. Results showed that adsorption of fluorescein-tagged ssDNA to GO resulted in fluorescence quenching and DNA was released from GO by adding complementary DNA. In addition, fluorescence restoration was increased through a polymerization reaction by the Klenow fragment in the presence of a fluorescein-attached template, GO, and primer. Gel electrophoresis was conducted to confirm the hybridization and DNA polymerization reactions on GO. © 2013, The Korean Society for Microbiology and Biotechnology.
키워드
Biosensor; Graphene oxide; Klenow fragment; Platform; complementary DNA; DNA polymerase; double stranded DNA; fluorescent dye; graphene oxide; oligomer; single stranded DNA; adsorption; article; enzyme activity; fluorescence analysis; gel electrophoresis; hybridization; polymerization; surface property
- 제목
- Real-time fluorescence assay of DNA polymerase using a graphene oxide platform
- 제목 (타언어)
- 산화 그래핀 플랫폼을 이용한 DNA 중합효소의 실시간 형광에세이
- 저자
- Gang, JongBack
- 발행일
- 2013-12
- 유형
- Article
- 저널명
- 한국미생물·생명공학회지
- 권
- 41
- 호
- 4
- 페이지
- 456 ~ 461