Vogesella oryzagri sp. nov., isolated from the rhizosphere of rice and in silico genome mining for the prediction of biosynthetic gene clusters

초록

A Gram- stain- negative, aerobic, rod- shaped, motile and flagellated novel bacterial strain, designated MAHUQ-64T, was isolated from the rhizosphere of rice. The colonies were observed to be creamy white- coloured, smooth, spherical and 0.5-1.1 mm in diameter when grown on Reasoner's 2A agar medium for 2 days. Strain MAHUQ-64T was able to grow at 10-40 degrees C, at pH 5.0-9.5 and in the presence of 0-2.0%NaCl (w/v). The strain was positive for both catalase and oxidase tests. The strain was positive for hydrolysis of L- tyrosine. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Vogesella and is closely related to Vogesella oryzae L3B39T (98.6% sequence similarity) and Vogesella facilis TTM-24T (98.2%). The novel strain MAHUQ-64T has a draft genome size of 3 827 146 bp (22 contigs), annotated with 3612 protein- coding genes, 74 tRNA and 4 rRNA genes. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-64T and its closest member V. oryzae L3B39T were 86.5 and 33.4%, respectively. In silico genome mining revealed several biosynthetic gene clusters in the genome of the novel strain MAHUQ-64T. The genomic DNA G+C content was determined to be 63.4 mol%. The predominant isoprenoid quinone was ubiquinone-8. The major fatty acids were identified as summed feature 3 (comprising C16:1co7c and/or C16:1co6c) and C16:0. Based on dDDH, ANI value, genotypic analysis and chemotaxonomic and physiological data, strain MAHUQ-64T represents a novel species within the genus Vogesella, for which the name Vogesella oryzagri sp. nov. is proposed, with MAHUQ-64T (=KACC 22245T=CGMCC 1.19000T) as the type strain.

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