Comprehensive safety assessment of Pediococcus acidilactici SY23 for potential probiotic use in inflammation management

초록

A comprehensive safety evaluation of P. acidilactici SY23 was conducted to assess its suitability as a probiotic strain with anti-inflammatory potential. Previous studies have reported anti-inflammatory activity of P. acidilactici SY23 in RAW 264.7 cells. In the present study, P. acidilactici SY23 exhibited no mucin degradation, gelatin liquefaction, urease or β-glucuronidase activity, or bile salt deconjugation. Biogenic amines and indoles were not detected, whereas D-lactate was produced at approximately 47 mM under the tested conditions. P. acidilactici SY23 also showed no β-hemolytic activity and no cytotoxicity toward Caco-2 cells. Antibiotic susceptibility testing showed that the minimum inhibitory concentrations were below EFSA cut-off values for all tested antibiotics. Whole-genome analysis further confirmed the absence of acquired antibiotic resistance and virulence genes. Collectively, these findings suggest that P. acidilactici SY23 may be a suitable probiotic candidate under the evaluated conditions.

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