Solitalea agri sp. nov., a new member of the genus Solitalea isolated from rhizospheric soil of a jujube tree

초록

A Gram- stain negative, aerobic, rod- shaped and creamy pink- coloured bacterium, designated MAHUQ- 68(T), was isolated from rhizospheric soil of a jujube tree. Colonies grew at 10-40 degrees C (optimum, 28 degrees C), pH 6.0-9.0 (optimum pH, 7.0) and in the presence of 0-1.5 % NaCl (optimum 0-0.5 %). Positive for both catalase and oxidase activity. Strain MAHUQ- 68(T) hydrolysed casein, starch, aesculin and l- tyrosine. Based on the results of phylogenetic analysis using 16S rRNA gene and genome sequences, strain MAHUQ-68(T) clustered together within the genus Solitalea. The closest members were Solitalea longa HR-AV(T) (98.8 % sequence similarity), Solitalea canadensis DSM 3403(T) (96.9 %) and Solitalea koreensis R2A36-4(T) (94.0 %). The genome of strain MAHUQ68(T) was 4 250 173 bp long with 68 scaffolds and 3 570 protein- coding genes. The genomic DNA G+C content of the type strain was 38.0 mol%. The average nucleotide identity and in silico DNA-DNA hybridization values between strain MAHUQ- 68(T) and its closest relatives were 72.0-81.4% and 19.8-24.3 %, respectively. The major cellular fatty acids were iso- C-15: 0 and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c). The main respiratory quinone was menaquinone- 7. The polar lipids comprised phosphatidylethanolamine, an unidentified aminolipid and four unidentified lipids. Based on these data, strain MAHUQ- 68(T) represents a novel species in the genus Solitalea, for which the name Solitalea agri sp. nov. is proposed. The type strain is MAHUQ- 68(T) (=KACC 22249(T)=CGMCC 1.19062(T)).

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