Nocardioides agri sp. nov., isolated from garden soil

초록

A novel bacterial strain, designated as MAH- 18T, was isolated from soil sampled in a flower garden. Cells of strain MAH- 18T were Gram- stain- positive, aerobic, motile, and rod- shaped. The colonies were beige in colour, smooth, and spherical when grown on Reasoner's 2A agar medium. Strain MAH- 18T grew at 20-40 degrees C, pH 6.0-8.0, and 0-1.0 % NaCl. Cells were able to hydrolyse aesculin, gelatin, and Tween 20. According to the 16S rRNA gene sequence comparisons, the isolate was determined to be a member of the genus Nocardioides and most closely related to Nocardioides pyridinolyticus OS4T (97.9 %), Nocardioides hankookensis DS-30T (97.9 %), Nocardioides aquiterrae GW-9T (97.6 %), Nocardioides soli mbc-2T (97.5 %), Nocardioides conyzicola HWE 2-02T (97.4 %), and Nocardioides mangrovi GBK3QG-3T (96.3 %). Strain MAH- 18T has a draft genome size of 4 788 325 bp (eight contigs), 4572 protein- coding genes, 46 tRNA, and three rRNA genes. The average nucleotide identity and digital DNA- DNA hybridization values between strain MAH- 18T and the closest type strains were 81.5-83.4 % and 24.4-25.8 %, respectively. In silico genome mining revealed several biosynthetic gene clusters in the genome of the novel strain MAH- 18T. The G+C content of the genomic DNA of strain was 72.2 mol% and the predominant isoprenoid quinone was MK- 8 (H4). The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and unknown phospholipids. The major cellular fatty acids were determined to be C16:0 iso and C17: 1 omega 6c. The DNA-DNA hybridization results and phenotypic, genotypic, and chemotaxonomic data demonstrated that strain MAH- 18T represents a novel species, for which the name Nocardioides agri sp. nov. is proposed, with MAH- 18T as the type strain (=KACC 19744T=CGMCC 1.13656T).

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