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Characterization of Xylanase from Bacillus agaradhaerens DK-2386 Isolated from Korean Soil
- Choi, J.-H.;
- Park, Y.-S.;
- Lee, H.;
- Bai, D.-H.
SCOPUS
0초록
The optimum conditions for the production of xylanase from Bacillus agaradhaerens DK-2386 have been previously investigated. In this study xylanase was purified by ammonium sulfate precipitation and CM-sepharose ion exchange chromatography. The molecular mass of the xylanase as determined by SDS-PAGE was 23 kDa in a form of monomeric enzyme. The optimum pH and temperature for xylanase activity was 6.0 and 60°C, respectively. Xylanase activity was increased by the addition of EDTA and then stabilized at 40°C for 24 h. The maximum xylanase activity was obtained when Birchwood xylan was used as a substrate and the Vmax and Km were 49,724 μmol/min and 6.08 mg/ml, respectively. © 2015, The Korean Society for Microbiology and Biotechnology.
키워드
- 제목
- Characterization of Xylanase from Bacillus agaradhaerens DK-2386 Isolated from Korean Soil
- 제목 (타언어)
- 토양으로부터 분리한 Bacillus agaradhaerens DK-2386 균주가 생산하는 Xylanase의 특성
- 저자
- Choi, J.-H.; Park, Y.-S.; Lee, H.; Bai, D.-H.
- 발행일
- 2015-12
- 유형
- Article
- 저널명
- 한국미생물·생명공학회지
- 권
- 43
- 호
- 4
- 페이지
- 330 ~ 335
- 언어
- KOR
- 출판사
- Korean Society for Microbiolog and Biotechnology
- 발행국가
- 대한민국
- 분량
- 6 페이지
- ISSN
- E 2234-1305
P 1598-642X