Deleterious effects of perfluorooctanesulfonate (PFOS) exposure on glucose and lipid metabolisms in the marine water flea Diaphanosoma celebensis

초록

Perfluorooctanesulfonate (PFOS) is recognized as a major persistent pollutant due to its high environmental stability and bioaccumulation potential. This study investigated the effects of PFOS exposures (30, 60, and 90 & micro;g/ L) on growth, reproduction, oxidative stress, and energy metabolism in the marine water flea Diaphanosoma celebensis. PFOS exposure inhibited growth and fecundity in a concentration-dependent manner, with significant suppression observed at 60 and 90 & micro;g/L. In addition to deleterious in vivo endpoints, the antioxidant enzymatic activities were significantly decreased, particularly at 90 & micro;g/L of PFOS, after reaching its peak at 60 & micro;g/L, suggesting that the antioxidant defense system may have reached its functional limit or been significantly compromised at the highest concentration. Lipid and glycogen analyses revealed concentration-dependent metabolic alterations. Specifically, at 30 & micro;g/L, reductions in both triglyceride and fatty acid levels suggested enhanced lipid utilization for energy production. In contrast, exposure to 90 & micro;g/L led to lipid accumulation and glycogen depletion, indicating impaired energy turnover. Gene expression analysis showed that CPT1A was upregulated at low PFOS concentrations, promoting fatty acid R-oxidation, whereas high PFOS concentrations upregulated G6Pase, MGAT, and DGAT while downregulating CPT1A and GYS, thereby favoring gluconeogenesis and lipid storage. This study demonstrates that PFOS induces concentration-dependent disruption of energy metabolism in D. celebensis, highlighting its potential ecological risks in aquatic environments.

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