Synergistic effects of bisphenol A and elevated temperature on lipid metabolism and fatty acid synthase in the marine rotifer Brachionus plicatilis

초록

This study examines how bisphenol A (BPA) and elevated temperature (30 degrees C) affect growth, reproduction, oxidative stress, and lipid metabolism in the marine rotifer Brachionus plicatilis. We found that 30 degrees C alone increased reproductive output but shortened lifespan, whereas 30 degrees C and exposure to BPA reversed reproductive gains and restored lifespan to 25 degrees C control levels. Reactive oxygen species levels, as well as superoxide dismutase and catalase activities, were highest at 30 degrees C plus BPA exposure, indicating synergistic oxidative stress. Analysis of the single-exon FASN gene in B. plicatilis, B. rotundiformis, and B. koreanus showed two NADB_Rossmann domains and conserved fatty acid synthesis sites, although B. plicatilis displayed unique loop and interdomain variations. Expression of FASN was elevated at 30 degrees C but reverted to near-control levels at 30 degrees C plus BPA, whereas FABP, MGAT, and DGAT were consistently upregulated. These results reveal how BPA and temperature interact to modulate survival, lipid metabolism, and potential structural adaptations in B. plicatilis.

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